Fig. 3
A Representative autoradiographic images of the hybridized with 35S-labeled oligodeoxynucleotide probes complimentary to AVP mRNA (A a, d), eGFP mRNA (A b, e), and CRH mRNA (A e, f) in the paraventricular nucleus (PVN) of AVP-eGFP transgenic rats. Sections were obtained 12 h after i.p. administration of saline (A a, b) or cisplatin (A d, e), and 6 h after i.p. administration of saline (A c) or cisplatin (A f). The highest signal intensity is indicated in black and the lowest signal intensity is indicated in white. Scale bar = 100 µm. AVP mRNA (B), eGFP mRNA (C), and CRH mRNA (D) were measured in the parvocellular division of the PVN 6 and 12 h after i.p. administration of saline or cisplatin in AVP-eGFP transgenic rats. Data are presented as the mean ± SEM (for each group at each time point, n = 12). *P < 0.05 versus saline-treated group. E Plasma concentrations of corticosterone 6 and 12 h after i.p. administration of saline or cisplatin in AVP-eGFP transgenic rats. Data are presented as the mean ± SEM (for each group at each time point, n = 12). *P < 0.05 versus saline-treated group