Fig. 3

Whole-cell patch-clamp recordings in TRPV6-expressing HEK293T cells. a Representative time trace of membrane currents from cells expressing WT or p.Gly291Ser with a − 60 mV holding potential. Divalent cation-free (DVF) bath solution-evoked sustained currents and 30 mM Ca²⁺-evoked transient currents were observed in both traces. b Current–voltage relationship currents in a. Red, gray and black dots in the traces indicate the points at which I–V curves were generated. c Statistical analysis of the peak amplitude of Ca²⁺-evoked currents. The currents for p.Arg390His were significantly smaller compared to those for WT (p = 0.013, n = 4, Mann–Whitney rank-sum test). Currents for p.Gly291Ser did not significantly differ from that for the wild type