Fig. 5

Overexpression of CLIC3 attenuated cell proliferation in KATOIII and NUGC-4 cells. a Expression level of CLIC3 in the membrane fractions of mock- and CLIC3-transfected KATOIII and NUGC-4 cells. Anti-CLIC3 antibody was used to detect CLIC3 protein (27 kDa). b Immunocytochemistry of KATOIII (left) and NUGC-4 cells (right) transfected with CLIC3 using anti-CLIC3 antibody (green). Cell nucleuses were stained with DAPI (blue). Scale bars, 10 μm. c Effects of CLIC3 expression on cell proliferation of KATOIII (left) and NUGC-4 cells (right). Increased cell numbers between 1st and 2nd countings (see Methods) are shown. Experiments were performed in triplicate. The bars represent the means ± SEM. *p < 0.05, **p < 0.01