Fig. 4

Knockdown of CLIC3 accelerated cell proliferation in MKN7 cells. a Transfection of Alexa 488-conjugated siRNA in MKN7 cells. b Expression level of CLIC3 in MKN7 cells transfected with negative control siRNA (si-NC) and CLIC3 siRNA (si-CLIC3). Anti-CLIC3 antibody was used to detect CLIC3 protein. Expression of β-actin (45 kDa) was used as a loading control. c Effects of CLIC3 expression on cell proliferation of MKN7 cells. Increased cell numbers at 48 h after replating are shown. Five independent experiments were performed. The bars represent the means ± SEM. **p < 0.01