Fig. 3

Electrophysiology in the CLIC3-expressing cells. a Expression of CLIC3 in the membrane fractions of mock (pcDNA4/His B vector)- and CLIC3 (CLIC3-pcDNA4 vector)-transfected HEK293T cells. Anti-CLIC3 and anti-Xpress antibodies were used to detect CLIC3 protein. b Immunocytochemistry of the mock (pcDNA4/His B vector)- and CLIC3 (CLIC3-pcDNA4 vector)-transfected HEK293T cells using anti-CLIC3 antibody (green; left) and anti-Xpress antibody (red; middle). Merged image is also shown (yellow; right). Cell nuclei was stained with DAPI (blue; right). Scale bars, 10 µm. c Representative traces of whole-cell currents obtained from mock (pIRES2-AcGFP1 vector)- and CLIC3 (CLIC3-pIRES2-AcGFP1 vector)-transfected HEK293T cells. d Current–voltage relationships of mock-transfected cells (black) and CLIC3-transfected cells (red). Each data point represents the means ± SEM of 10 and 15 experiments, respectively. **p < 0.01. e Current–voltage relationships of CLIC3-transfected cells exposed to standard bathing solution (control: red) and the low Cl⁻ bathing solution (blue). Each data point represents the means ± SEM of 11 experiments. *p < 0.05, **p < 0.01. f Current–voltage relationships of CLIC3-transfected cells in the absence (red) and presence (blue) of 100 µM NPPB. Each data point represents the means ± SEM of 10 experiments. *p < 0.05, **p < 0.01. g Current–voltage relationships of gastric cancer MKN7 cells endogenous expressing CLIC3 in the absence (red) and presence (blue) of 100 µM NPPB. Each data point represents the means ± SEM of 9 experiments. **p < 0.01