Fig. 1

Expression of CLIC3 in human gastric cancer cells. a Tissue microarray (TMA) analysis using anti-CLIC3 antibody in the tumor of 107 patients with gastric cancer. Scale bar, 5 mm. b Enlarged images of the TMA samples judged as “CLIC3-high” (left and middle) and “CLIC3-low” (right). Red arrowheads in left and middle panels indicate expression of CLIC3 in apical side. Scale bars, 100 µm. c Representative images of Western blotting of gastric cancer tissues (T) and adjacent non-cancer tissues (N) in CLIC3-high (#1 and #2) and CLIC3-low (#3 and #4) samples. #1, 85F, non-solid type poorly differentiated adenocarcinoma, T4aN2M0, StageIIIA; #2, 58 M, signet-ring cell carcinoma, T4aN3aM0, StageIIIB; #3, 67 M, signet-ring cell carcinoma, T1bN0M0, StageIA, #4, 69 M, solid type poorly differentiated adenocarcinoma, T1bN0M0, StageIA. Clinicopathological explanation of specimens is given in the footnote of Table 1. Single band of CLIC3 was observed at 27 kDa. d Expression levels of CLIC3 in the membrane fractions of five gastric cancer cell lines. Expression of β-actin (45 kDa) was used as a loading control. e Immunocytochemistry of MKN7 cells using anti-CLIC3 antibody (green). Cell nucleuses were stained with DAPI (blue). XY and XZ images are shown. Scale bars, 10 µm