Fig. 4

Western blot analysis of total phospholamban (PLB), phosphorylated phospholamban on Ser16 (p-PLB ^Ser16) and Thr17 (p-PLB^Thr17), and GAPDH in LV tissues of the presence or absence of Cap or CPZ at 37 °C or 42 °C. Representative data of total PLB, p-PLB ^Ser16, and p-PLB^Thr17 (a). Comparison of the mean protein levels of total PLB/GAP (b), p-PLB^Ser16/PLB (d), and p-PLB^Thr17/PLB (c). Values are mean ± SD of 5–6 LV tissues for each experimental group. *p < 0.05 vs. 32 °C,^†p < 0.05 vs. 42 °C