Fig. 5

The Mfn2–Yap signaling pathway affects calcium overloading and oxidative stress in N2a cells. a–d Immunofluorescence assay was used to observe the alterations in store-operated calcium entry (SERCA) and the inositol trisphosphate receptor (IP3R). siRNA against Yap (si-Yap) and adenovirus-loaded Mfn2 (Ad-Mfn2) were transfected into N2a cells. e, f Cellular calcium was labeled with Fure-2AM and then the fluorescence intensity of calcium was measured as a reflection of calcium overloading. g, h Reactive oxygen species (ROS) production was measured by the immunofluorescence assay. siRNA against Yap and adenovirus-loaded Mfn2 were transfected into N2a cells. i–k ELISA assay was used to observe the changes in cellular antioxidants. Mfn2 overexpression attenuated TNFα-mediated ROS overproduction and this effect could be negated by Yap deletion. Data are presented as the mean of three replications with the SEM. Asterisk above columns linked by horizontal line indicates a significant difference between treatments at p < 0.05. Cont Control