Fig. 2

Overexpression of Mfn2 abolishes TNFα-mediated apoptosis in mouse neuroblastoma N2a cells. a–c Proteins were isolated from N2a cells and the expressions of Mfn2 and Yap were determined by western blotting. Adenovirus-loaded Mfn2 (Ad-Mfn2) was transfected into N2a cells to overexpress Mfn2. d Cell viability was determined by the MTT assay. Overexpression of Mfn2 reversed cell viability in TNFα-treated N2a cells. e An enzyme-linked immuosorbent assay (ELISA) was used to observe the changes in caspase-3 in response to TNFα treatment and/or Mfn2 overexpression. Mfn2 overexpression reduced TNFα-mediated capsase-3 upregulation. f, g The immunofluorescence assay was used to observe the changes in caspase-3 in N2a cells. h–j The knockdown efficiency of small interfering RNA (siRNA) against Yap (si-Yap) was determined using western blotting. Mfn2 expression was also measured in response to Yap siRNA transfection. Data are presented as the mean of three replications with the SEM. Asterisk above columns linked by horizontal line indicates a significant difference between treatments at p < 0.05. Cont Control