Fig. 1

Tumor necrosis factor alpha (TNFα) reduces the levels of mitofusin 2 (Mfn2) and Yes-associated protein (Yap) in mouse neuroblastoma N2a cells. a N2a cells were treated with TNFα and then the viability of the treated N2a cells was determined using the MTT assay. TNFα treatment reduced the viability of the N2a cells. b–d Proteins were isolated from N2a cells and then western blotting was used to confirm alterations in the expression of Yap and Mfn2. e–h Immunofluorescence assay was used to analyze the expression of Mfn2 and Yap in N2a cells. TNFα treatment reduced the levels of Mfn2 and Yap in N2a cells. Data are presented as the mean of three replications with the standard error of the mean (SEM). Asterisk above columns linked by horizontal line indicates a significant difference between treatments at p < 0.05. Cont Control, DAPI 4′,6-diamidino-2-phenylindole, GAPDH glyceraldehyde 3-phosphate dehydrogenase (internal control)