Fig. 5

Currents activated by pregnenolone sulfate or nifedipine in Xenopus oocytes expressing mouse TRPM3α2, TRPM3γ2, or TRPM3γ3. a–d Representative traces of endogenous (mock, a), TRPM3α2 (b), TRPM3γ2 (c), or TRPM3γ3 (d) currents activated by treatment of Xenopus oocytes with 1–300 μM pregnenolone sulfate. The membrane potential was held at − 60 mV. e Representative current–voltage curves of the currents by treatment of distilled water-injected Xenopus oocytes (mock) or Xenopus oocytes expressing TRPM3α2, TRPM3γ2, or TRPM3γ2 with 1, 10, and 100 μM pregnenolone sulfate (PS). f Dose–response profiles of currents in Xenopus oocytes expressing TRPM3α2, TRPM3γ2, or TRPM3γ3 activated by pregnenolone sulfate. Mock indicates the currents by pregnenolone sulfate in distilled water-injected Xenopus oocytes. Hill coefficients were 1.3 ± 0.1 (TRPM3α2), 1.4 ± 0.2 (TRPM3γ2) and 1.5 ± 0.2 (TRPM3γ3). Each symbol represents the mean ± SEM of 9–11 oocytes. Statistical significance was assessed using ANOVA followed by a two-tailed multiple t test with Bonferroni correction. *P < 0.05, **P < 0.01, versus DW, ^#P < 0.05, ^##P < 0.01, versus TRPM3α2. g–i Representative traces of endogenous (mock, g), TRPM3α2 (h) or TRPM3γ2 (i) currents activated by 100 μM nifedipine in Xenopus oocytes. The membrane potential was held at − 60 mV. Pregnenolone sulfate (PS, 100 μM) was applied in the end. j Representative current–voltage curves of the currents by 100 μM nifedipine in distilled water-injected Xenopus oocytes (mock) or Xenopus oocytes expressing TRPM3α2, TRPM3γ2, or TRPM3γ3 with 100 μM nifedipine (Nif). k Comparison of peak nifedipine (100 μM)-evoked currents in distilled water-injected Xenopus oocytes (mock), and Xenopus oocytes expressing TRPM3α2, TRPM3γ2, or TRPM3γ3. Each column represents the mean ± SEM of 5–10 oocytes. Statistical significance was assessed using ANOVA followed by a two-tailed multiple t-test with Bonferroni correction. **P < 0.01, versus DW, ^##P < 0.01, versus TRPM3α2