Fig. 4
Changes in the serum glucose levels during an intraperitoneal insulin tolerance test (IPITT). Female rats were assigned to the following regime for 6 weeks. Ad libitum supply of water and food (normal-control, WAFA), water supply for 1 h per day with ad libitum food intake (WRFA), and ad libitum water supply plus pair feeding of food to as much as WRFA (WAFR). Rats in WR-WA had water restriction for 3 weeks and then ad libitum water supply with ad libitum food intake for 3 weeks. An IPITT was conducted by intraperitoneally injecting insulin (1 U/kg body weight) after a 6 h fast and serum glucose levels were measured every 15 min. The averages of the areas under the curve (AUC) of glucose (B) were calculated for the first part (0–40 min) and the second part (40–120 min) of the IPITT. Each bar and error bar represents mean ± SD (n = 10). *Significantly different among all groups by one-way ANOVA at p < 0.05 (n = 10). a, b, cBars with a different alphabet differ significantly by Tukey’s test at p < 0.05