Fig. 7

SFXN2-KO cells display increased sensitivity to iron. a Control and SFXN2-KO cells were seeded in 96-well plates at a density of 5000 cells/well. SFXN2-KO cells grew significantly slower than control cells. n = 7–8. ****p < 0.0001. b Control and SFXN2-KO cells were treated with the indicated concentrations of ferric ammonium citrate (FAC) for 24 h. Growth of SFXN2-KO cells was slowed more than that of control cells in the presence of FAC. n = 7–8. *p < 0.05, **p < 0.01, ****p < 0.0001. c Control and SFXN2-KO cells were treated with 10 μM erastin for 24 h and then observed under a microscope. The density of SFXN2-KO cells was markedly reduced upon erastin treatment. Bar = 200 μm. d Cell viability was measured using the WST-8 reagent. Erastin treatment decreased the viability of SFXN2-KO cells significantly more than that of control cells. n = 7–8. ****p < 0.0001. e Control and SFXN2-KO cells were stained with trypan blue, which labels dead cells. Erastin treatment increased the number of SFXN2-KO cells that were labeled with trypan blue. Bar = 200 μm. f Cell death was assessed by measuring LDH activity in the culture medium. Erastin treatment significantly increased LDH activity in the culture medium of SFXN2-KO cells. ****p < 0.0001