Fig. 6
Effects of chemical cleavage for removal of glycosylation on CaV3.1-ICa.T. Changes of the activation (G/Gmax) and the steady-state inactivation (availability) curves were evaluated by a non-specific glycosidase PNGase F in the cell-culture medium prior to the electrophysiological experiments for 2 h. Representative current traces with vehicle or PNGase F (a), group data for current (I)-voltage (V) relationship (b), and changes of the activation (G/Gmax) and the steady-state inactivation (Availability) curves as group data (c). The number of cells is indicated in parentheses. Values represent the mean ± SE