Fig. 3
Concentration-dependent effects of tunicamycin on CaV3.1-ICa.T. HEK-CaV3.1 cells were incubated for 24 h with vehicle or tunicamycin at the concentration of 10−6 g/l, 10−5 g/l, 10−4 g/l, 10−3 g/l, or 10−2 g/l. a Current (I)-voltage (V) relation were constructed by normalizing each ICa.T to that of the maximum inward current with vehicle (I/Iveh). b Dose–response relationship of tunicamycin on ICa.T was evaluated by a pulse protocol composed of a holding potential of − 100 mV to a test potential for the maximum current (− 30 or − 25 mV) at the stimulation frequency of 0.2 Hz. Data were fitted by the Hill equation yielding IC50 value of 2.01 × 10−3 g/l. Values represent the mean ± SE. *p < 0.01 vs. vehicle