Fig. 6

Pain-related and itch-related behaviors induced by FK506 in mice. a Time spent by wild-type (WT) mice licking or biting in the 10 min following intraplantar injection of vehicle, 6.7 nmol FK506 or 20 nmol FK506. Each column represents the mean + SEM from 7 to 9 mice. Statistical analysis was performed using ANOVA followed by a two-tailed multiple t test with Bonferroni correction. **, P < 0.01 vs. vehicle; #, P < 0.05 vs. 6.7 nmol. b Time spent by WT or TRPA1 knockout (TRPA1KO) mice licking or biting in the 10 min following intraplantar injection of vehicle or 20 nmol FK506. Each column represents the mean + SEM from 8 or 9 mice. Statistical analysis was performed using ANOVA followed by a two-tailed multiple t test with Bonferroni correction. **, P < 0.01 vs. vehicle; #, P < 0.01 vs. WT. c Time spent by WT or TRPM8 knockout (TRPM8KO) mice licking or biting in the 10 min following intraplantar injection of vehicle or 20 nmol FK506. Each column represents the mean + SEM from 6 to 9 mice. Statistical analysis was performed using ANOVA followed by a two-tailed multiple t test with Bonferroni correction. *, P < 0.05, **, P < 0.01 vs. vehicle. d The number of incidents of scratching following intradermal injection of vehicle or 20 nmol FK506 into the back skin over a 15-min period. Each column represents the mean + SEM from 6 mice