Fig. 5

Increases in intracellular Ca²⁺ concentration induced by FK506 in mouse dorsal root ganglion neurons. a Representative traces of changes in intracellular Ca²⁺ concentrations ([Ca²⁺]_i) induced by 30 μM FK506 in mouse dorsal root ganglion (DRG) neurons. Mouse DRG neurons were categorized by 100 μM AITC and 1 μM capsaicin (Cap). Neurons were determined by 50 mM KCl and cell viability was confirmed by 5 μM ionomycin (Iono). Y-axis: fluorescence intensity of Fluo-4. b Classification of mouse DRG neurons depending on the response to 30 μM FK506 and 100 μM AITC. A total of 186 neurons were obtained from three mice. The X-axis indicates the number of cells. c [Ca²⁺]_i increases induced by 30 μM FK506 were almost abolished in the absence of extracellular Ca²⁺ (Ca (−)). d [Ca²⁺]_i increases induced by 30 μM FK506 were inhibited by 3 μM A967079, a TRPA1 antagonist, in the mouse DRG neurons responded to 100 μM AITC