Fig. 4

FK506 activates mouse TRPA1. a A representative trace of whole-cell patch-clamp recording in a HEK293T cell expressing mouse TRPA1 (mTRPA1) exposed to 55 μM FK506 (black bar) and 100 μM AITC (white bar). Membrane potential was held at − 60 mV and ramp pulses from − 100 to +100 mV were applied every 3 s. Insets indicate the representative I–V curves of the mTRPA1 currents in the absence (a) or presence (b) of 55 μM FK506. b Summary of the peak currents at − 60 mV induced by 55 μM FK506 in mTRPA1- or mock-transfected HEK293T cells. Each column represents the mean + SEM from 8 to 9 cells. Statistical significance was assessed using Student’s t test. **; P < 0.01 vs. mock