Fig. 2

FK506-induced inward currents were not observed in HEK293T cells expressing human TRPV1 or TRPM8. a A representative trace of whole-cell patch-clamp recording in HEK293T cells expressing human TRPV1 (hTRPV1) exposed to 55 μM FK506 (black bar) and 1 μM capsaicin (white bar). Membrane potential was held at − 60 mV and ramp-pulses from − 100 to + 100 mV were applied every 3 s. b A representative trace of whole-cell patch-clamp recording in HEK293T cells expressing human TRPM8 (hTRPM8) exposed to 55 μM FK506 (black bar) and 500 μM menthol (white bar). Membrane potential was held at − 60 mV and ramp pulses from − 100 to +100 mV were applied every 3 s