Fig. 2

Effect of naloxone on IPSCs in LC neurons of brain slices from morphine-treated rats. a Sample traces of eIPSCs at a holding potential of − 70 mV, in the absence (baseline) and presence of naloxone (1 µM, NLX). b Naloxone significantly decreased eIPSC amplitude (paired two-tailed Student’s t test, *P < 0.05, n = 8). c Sample traces of sIPSCs in the absence (baseline) and presence of naloxone. d Left, naloxone decreased sIPSCs frequency in LC neurons of morphine-treated rats (paired two-tailed Student’s t test, *P < 0.05, n = 8). Right, cumulative probability plots of sIPSCs inter-event interval in the absence (baseline) and presence of naloxone in LC neurons of morphine-treated rats. e Left, naloxone application did not change the sIPSCs amplitude of LC neurons of morphine-treated rats. Right, cumulative probability plots of sIPSCs amplitude in the absence (baseline) and presence of naloxone in LC neurons of morphine-treated rats