Fig. 8

Intersectin-1 is required for the formation of actin patches and the recruitment of PI3K-C2β to the clathrin-coated structures. a, b Confocal microscopy coupled with SRRF-Stream mode images of cells transfected with scrambled and ITSN1-specific siRNA. Double immunostaining with anti-ITSN1 (green) (a) or anti-PI3K-C2β (green) (b) antibodies and with Alexa Fluor 594-conjugated phalloidin (red, left panels) or anti-CHC antibody (red, right panels). Nuclei were stained with DAPI (blue). The region outlined by the dotted box is shown at higher magnification. White arrowheads in SRRF view of the merged image indicate the co-localization of endogenous ITSN1 (a) or PI3K-C2β (b) with F-actin or clathrin-coated structures. c, d Co-localization index of PI3K-C2β and F-actin (c) or CHC (d). Data are presented as the median and IQR. Asterisks indicate statistical significance between the indicated groups: *p < 0.05, **p  < 0.01, ***p < 0.001