Fig. 7

Overexpression of PI3K-C2β but not PI3K-C2α enhances dextran uptake in the presence of intersectin-1 (ITSN1) overexpression (a, c). Super-resolution microscopic images of cells. Cells were transfected with the expression vectors of mCherry or mCherry-C2β or C2α (red) and GFP or GFP-ITSN1 (green). The cells were then subjected to Alexa Fluor 647-dextran (purple). Nuclei were stained with DAPI (blue). Merged images of the mCherry and GFP signals in the boxed regions are shown in the lower-right panels of the middle (GFP) row, with white arrowheads (yellow-colored dots) indicating the overlap of red and green signals. Yellow asterisks indicate nuclei of vectors-transfected cells, the hashtags indicate the nuclei of non-transfected cells. b, d Upper, Average number of dextran⁺ vesicles per cell; lower, average number of mCherry-C2β⁺ (b) or mCherry-C2α⁺ (d) vesicles per cell. Data are presented as the median and IQR. Asterisks indicate statistical significance between the indicated groups: *p < 0.05, **p  < 0.01, ***p < 0.001; ns not significant