Fig. 4

Immunofluorescent staining of endogenous PI3K-C2β in HUVECs. Cells were double-immunostained with anti-PI3K-C2β (green) and Alexa Flour 594-conjugated phalloidin (red), anti-CHC (red), anti-EEA1 (red) or anti-LC3B (red), and imaged by confocal microscopy coupled with SRRF-Stream mode. Nuclei were stained with DAPI (blue). Scale bar: 20 μm. White arrowheads in SRRF views indicate the co-localization of endogenous PI3K-C2β with each organelle marker