Fig. 6
From: Sex differences in forkhead box O3a signaling response to hindlimb unloading in rat soleus muscle
Myostatin signaling transducers and mRNA expression. Myostatin (a), follistatin (b), phosphorylation ratio of Smad2 (Ser465/467)/Smad3 (Ser423/425) (c) and representative blots (d), and TGF-β (e), Smad2 (f) and Smad3 (g) mRNA expression. Samples were collected before (0d), and 1 day (1d) and 7 days (7d) after hindlimb unloading. Values are mean ± SE; n = 6 per time point. β-Actin was used as a loading control and mRNA expression was normalized to that of 18S mRNA. The results of two-way analysis of variance are displayed. *p < 0.05 vs. males at each time point