Fig. 4
Inhibition of phospholipase C (PLC) did not affect the increase in [Ca2+]i induced by Lys-[Des-Arg9]BK in TG neurons. a Representative trace of 10 nM Lys-[Des-Arg9]BK-induced [Ca2+]i increases (white boxes at top of graph) in the absence (white segments of lower horizontal bar) or the presence (gray segments of lower horizontal bar) of extracellular Ca2+ (2.0 mM). The increase in [Ca2+]i induced by 10 nM Lys-[Des-Arg9]BK was not affected by the application of 100 nM U73122 (black bar at top of graph) in the absence of external Ca2+. Gray box on the upper-right side of the graph indicates an application timing of high-extracellular-K+ (50 mM) solution. b Summary bar graph showing [Ca2+]i increases following the application of 10 nM Lys-[Des-Arg9]BK (upper and middle bar), and following the application of 10 nM Lys-[Des-Arg9]BK with 100 nM U73122 (lower bar), with or without external Ca2+ (2.0 mM) (gray or white boxes on the right side of graph, respectively). c Representative trace of 10 nM Lys-[Des-Arg9]BK-induced [Ca2+]i increases (upper white boxes) in the presence (lower gray bar) of extracellular Ca2+ (2.0 mM). The increase in [Ca2+]i induced by 10 nM Lys-[Des-Arg9]BK was not affected by the application of 1 μM xestospongin C (Xest-C; black bar at top of graph). The gray box on the upper-right side of graph indicates the timing for application of high-extracellular-K+ (50 mM) solution. d Summary bar graph showing [Ca2+]i increases following the application of 10 nM Lys-[Des-Arg9]BK (upper and lowermost bar) and following the application of 10 nM Lys-[Des-Arg9]BK with 1 μM xestospongin C (middle bar), with external Ca2+ (2.0 mM) (vertical gray bar). Each bar denotes the mean ± SE of six experiments in b and five experiments in d, respectively. In b and d, the statistical significance between bars (shown by solid lines) is indicated by asterisks: *p < 0.05