Fig. 1
Range in diameter of primary-cultured trigeminal ganglion (TG) cells and increases in the intracellular free Ca2+ concentration ([Ca2+]i) in primary-cultured TG neurons following the application of Lys-[Des-Arg9]BK, a B1 receptor agonist. a Distribution of the cell body diameter of Lys-[Des-Arg9]BK-responding (white segment of bars) and -non-responding TG neurons (black segment of bars) are shown for 127 neurons in total (from 16 experiments). b Example of transient [Ca2+]i increases following the application of 10 nM Lys-[Des-Arg9]BK (white boxes at top of graph) in the presence (gray segments of lower horizontal bar) or absence (white segment of lower horizontal bar) of extracellular Ca2+ (2.0 mM). Gray square on the upper-right side of graph indicates the timing of an application of a high-extracellular-K+ (50 mM) solution. c Summary bar graph showing [Ca2+]i increases following applications of 10 nM Lys-[Des-Arg9]BK. Upper, second from the top, and lowermost bar show the values following the first, second and third application of 10 nM Lys-[Des-Arg9]BK, respectively, in the presence of external Ca2+ (2.0 mM) (gray boxes on right side of graph). Third and fourth bar from the top show the mean values for the increase in [Ca2+]i following the first and second application of 10 nM Lys-[Des-Arg9]BK, respectively, in the absence of external Ca2+ (white box on the right side of graph). Each bar denotes the mean ± standard error (SE) of 16 experiments. Statistical significance between bars (shown by solid lines) is indicated by asterisks: *p < 0.05