Fig. 5

Matrine regulates mitochondrial fission via the Mst1-JNK pathways. a–c Western blotting was performed to analyse the expression of Mst1 and JNK phosphorylation in response to matrine. d, e The siRNA against Mst1 was transfected into HepG2 cells. The knockdown efficiency was verified via western blotting. f–h The immunofluorescence assay for JNK phosphorylation and Mst1 expression in the presence of matrine. The siRNA was transfected into matrine-treated cells to inhibit the activation of Mst1. i, j Mitochondrial fission was monitored using an immunofluorescence assay. The average length of mitochondria was recorded. Experiments were repeated 3 times (n = 3), and data are shown as the mean ± SEM. *P < 0.05 vs the control group. Cont control, Mat matrine