Fig. 1

Matrine induces HepG2 and Huh7 cell death. a, b An LDH release assay was performed to analyse cell death. Different doses of matrine were added into the medium of HepG2 cells and Huh7 cells. c–e Cell death was quantified via TUNEL assays. The number of TUNEL-positive cells was monitored. f–k Western blotting was used to analyse the expression of caspase-3 and PARP. Experiments were repeated 3 times (n = 3), and data are shown as the mean ± SEM. *P < 0.05 vs the control group. Cont control, Mat matrine