Fig. 1

Effect of GLP-1 on amylase release. a Isolated pancreatic acini were incubated with or without GLP-1 (25 nM) for the indicated times. b Acini were incubated with or without 8-bromo-cAMP (1 mM) for 15 min. c, d After pretreatment with H89 (10 μM) for 10 min (c) or cAMPS-Rp (15 μM) for 15 min (d), acini were incubated with or without GLP-1 (25 nM) for 15 min. Amylase release was expressed as the percentage of the total amylase activity. Values are shown as means ± SE from three independent experiments. The asterisks indicate a significant difference (P < 0.05) compared with the corresponding period for the control (a) or the control in the absence of 8-bromo-cAMP (b) or GLP-1 (c, d)