Fig. 4

Effect of ambient temperature on proliferation of brown adipocyte progenitors in interscapular fat of Syrian hamsters. Interscapular fat tissues were obtained from 10-day-old Syrian hamsters bred at 23 °C or 30 °C. a Representative images of the interscapular fat sections stained with antibodies against Ki67 (red) and MCT1 (green). Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar 100 µm. b The proportion of cells which stained double positive for Ki67 and MCT1 antibodies in total MCT1-positive cells was measured (mean ± SE, n = 4 per group, Student’s t test). Asterisk indicates significant difference at P  < 0.05 compared to the 23 °C group (colour figure online)