Fig. 1
Phase contrast and MQAE fluorescence images of airway ciliary cells. The images in A,a, A,b, B,a and B,b were obtained from the same optical focal plane of a cell. Experiments were carried out in the Cl−-free NO3− solution. A Hypo-osmotic stress (− 30 mM NaNO3) condition: a, c Phase contrast image (a) and MQAE fluorescence image (c) just before the application of the hypo-osmotic stress; b, d phase contrast image (b) and MQAE fluorescence image (d) 10 min after the application of the hypo-osmotic stress. The white line shows the outline of the cell. The application of the hypo-osmotic stress induced cell swelling, but there was little change in MQAE fluorescence intensity. B Hyper-osmotic stress (+ 30 mM NaNO3) condition: a, c Phase contrast image (a) and MQAE fluorescence image (c) before the application of the hyper-osmotic stress; b, d phase contrast image (b) and MQAE fluorescence image (d) 10 min after the application of the hyper-osmotic stress. The white line shows the outline of the cell. The application of hyper-osmotic stress induced cell shrinkage, but there was little change in MQAE fluorescence intensity