Table 1 Primers used in the bait vector construction
Construct | Sense primer | Antisense primer |
|---|---|---|
SMCT1-CTwt | 5′-CGGAATTCACAGGAGGAAGAAAACAG-3′ | 5′-CATCTCGAGTCACAACAGGAGTCCCATTG-3′ |
SMCT1-CTd3 | 5′-CGGAATTCACAGGAGGAAGAAAACAG-3′ | 5′-CATCTCGAGCCCATTGCTCTTGCCACTCTCATG-3′ |
SMCT1-T608A | 5′-CGGAATTCACAGGAGGAAGAAAACAG-3′ | 5′-CATCTCGAGTCACAACAGAGCCCCATTGCTCTTG-3′ |
SMCT1-L610A | 5′-CGGAATTCACAGGAGGAAGAAAACAG-3′ | 5′-CATCTCGAGTCACGCCAGAGTCCCATTGCTCTTG-3′ |
SMCT2-CTwT | 5′-GCGAATTCCTGGCTTTTGGTCTAAGAAG-3′ | 5′-CTCCTCGAGTTAGAAATGGGTAGTCTC-3′ |
SMCT2-d3 | 5′-GCGAATTCCTGGCTTTTGGTCTAAGAAG-3′ | 5′-CTCCTCGAGTTAAGTCTCAAATGCCATATTG-3′ |
SMCT2-T616A | 5′-GCGAATTCCTGGCTTTTGGTCTAAGAAG-3′ | 5′-CTCTTCGAGTTAGAAATGGGCATGCTCAAATGC-3′ |
SMCT2-T618A | 5′-GCGAATTCCTGGCTTTTGGTCTAAGAAG-3′ | 5′-CTCTTCGAGTTAGGCATGGGTATGCTCAAATGC-3′ |