Fig. 2

Interaction of single PDZ domain of PDZK1 with C-termini of SMCT1 and SMCT2 in yeast two-hybrid system. a The wild-type SMCT1 C-terminus bait interacts with prey containing either the first or third PDZ domains of PDZK1 (PDZ1, PDZ3). The specificity of the prey containing a single PDZ domain of PDZK1 for the SMCT1 bait was confirmed by the absence of growth associated with the PEPT2 d3 mutant baits. b The wild-type SMCT2 C-terminus bait interacts with prey containing either the first, second or fourth PDZ domains of PDZK1 (PDZ1, PDZ2, PDZ4). The specificity of the prey containing a single PDZ domain of PDZK1 for the SMCT2 bait was also confirmed by the absence of growth associated with the PEPT2 d3 mutant baits. *Stop codon, A alanine mutation