Fig. 1

Specificity of PDZK1 interaction with C-termini of SMCT1 and SMCT2 in yeast two-hybrid system. a PDZK1 specifically interacted with the wild-type SMCT1 C-terminus but not with the C-terminal mutants T608A, L610A and d608-610 (d3) of SMCT1. b PDZK1 specifically interacted with the wild-type SMCT2 C-terminus but not with the C-terminal mutants T616A, F618A and d616-618 (d3) of SMCT2. pJG4-5 with PDZK1 cDNA expression cassette is under the control of the GAL1 promoter, such that library proteins are expressed in the presence of galactose (Gal) but not glucose (Glu). The system used for the yeast two-hybrid screen includes the reporter genes LEU2 and GFP, which replace the commonly used classical lacZ gene and allow a fast and easy detection of positive clones with long-wave UV. The results from the growth assay and GFP fluorescence are indicated on the right