Fig. 2

Histological method for identifying MD subnuclei. a Photomicrograph of the medial thalamus in a wet unstained frozen section. b The same section after AChE histochemical staining. Note MDc is fiber-rich and can be clearly distinguished in the wet freshly prepared section in a and the MDl is AChE-rich and clearly distinguishable in the AChE stained section in b. C MDc, ILN intralaminar nuclei, M MDm, L MDl. Calibration bar is 1 mm