Table 4 Primers used for the pyrosequencing assay
Gene | Amplification site | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | *Sequencing primer (5′ to 3′) |
|---|---|---|---|---|
NFKB2 | −1331 to −1153 | GGGTTTAGGGTAGGGAAAGTTTTT | Biotin-CCCCCCCTAAATAAACCTATCCCCTCT | −1185-TTTAGGAGGGGGGAAT |