Fig. 4
Expression level of PINK1/parkin-mediated mitophagy signaling proteins of SN in MPTP mice after treadmill exercise. Representative western blot showing mitochondrial PINK1 and parkin levels in the SN for all groups of mice are presented. a, c, d Mitochondrial PINK1 and parkin levels of SN were increased in MPTP mice compared with C57BL mice, and the levels of those proteins in the SN of MPTP mice were decreased after treadmill exercise. Tom20 protein was probed as an internal control. Representative western blot showing p62, LC3-II/LC3-I ratio in the SN for all groups of mice is presented. b, e, f p62 and LC3-II/LC3-I ratio were increased in MPTP mice compared with C57BL mice, and the levels of those proteins in the SN of MPTP mice were decreased after treadmill exercise. β-actin was probed as an internal control. Six mice per C57BL mice and MPTP mice subgroups were assayed in triplicate in western blot analysis. LSD post hoc test after ANOVA. Values are mean ± SEM. Saline control (CON), MPTP treatment with probenecid (MPTP), and MPTP treatment with probenecid plus treadmill exercise (MPTP-TE)