Fig. 3
Expression level and immunohistochemical analysis of TH in the substantia nigra of MPTP mice after treadmill exercise. Representative western blot showing TH levels for all groups of mice in the substantia nigra (SN) are presented. a, b TH was decreased in MPTP mice compared with C57BL/6 mice, and the levels of TH in MPTP mice were increased after treadmill exercise. β-actin was probed as an internal control. Immunohistochemical analysis of 40-μm-thick sections immunolabeled with anti-TH antibody. c Note that photomicrographs reveal that TH expression was notably enhanced in the SN region in MPTP mice whereas the enhanced expression was suppressed after treadmill exercise. d Number of TH-positive neurons. Values are expressed to 100% for levels of C57BL mice. Six mice per C57BL mice and MPTP mice subgroups were assayed in triplicate in western blot analysis and four mice per C57BL mice and MPTP mice subgroups were assayed in triplicate in immunohistochemical assay. Scale bars: 100 μm. LSD post hoc test after ANOVA. Values are mean ± SEM. Saline control (CON), MPTP treatment with probenecid (MPTP), and MPTP treatment with probenecid plus treadmill exercise (MPTP-TE)