Fig. 2

Effects of hypergravity and VL on the expression of myogenic differentiation genes in the soleus muscle of mice. Total RNA was extracted from the soleus muscle of mice treated with VL or sham surgery after exposure to 1 or 2 g for 2 weeks (a) and 8 weeks (b). Then, the level of MyoD, Myf5, myogenin, myosin heavy chain-I (MHC-I), atrogin-1, MuRF1 or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was assessed by real-time PCR analysis. Data are expressed relative to the levels of GAPDH. The data represent the mean ± SEM of 6 (a) and 4 (b) tissues in each group. **P < 0.01