Fig. 1

The number of Fos-like immunoreactivity (LI) cells in the supraoptic nucleus (SON) (A-a), the paraventricular nucleus (PVN) (A-b), the area postrema (AP) (A-c), the medial nucleus of the solitary tract (mNTS) (A-d) and the caudal NTS (cNTS) (A-e) after intraperitoneal (ip) administration of cisplatin (A) in rats. Colocalization of Fos-LI (brown in round structures) and OXT-LI (violet in spindle-shaped structures) in the SON (B-a–d) and the PVN (C-a–d). B-b, d, C-b, d enlargements from the boxed areas in B-a, c and C-a, c. Black arrowheads indicate colocalization of nuclear Fos-LI and OXT-LI. White arrowheads indicate OXT-LI without Fos-LI. The asterisks indicate Fos-LI without OXT-LI. The number of Fos-expressing in OXT-LI cells in the SON (B-e) and the PVN (C-e). Values represent the mean ± SEM (each group, n = 6). *p < 0.05 compared with the vehicle group. OC optic chiasma; 3V third ventricle. Scale bars 100 μm