Table 1 Primer pairs used for detection of mRNAs
From: Effect of lipopolysaccharide on circadian clock genes Per2 and Bmal1 in mouse ovary
Genes | Primer sequence | Size (bp) | GeneBank accession no. |
|---|---|---|---|
Per2 | Forward: 5′-GGCACATCTCGGGATCG-3′ Reverse: 5′-GAGCAGAGGTCCTCGCC-3′ | 112 | NM_011066 |
Bmal 1 | Forward: 5′-GGAGAAGGTGGCCCAAA-3′ Reverse: 5′-AGGCGATGACCCTCTTA-3′ | 135 | NM_001243048 |
CYP19 | Forward: 5′-CATGGTCCCGGAAACTGTGA-3′ Reverse: 5′-CTAGTAGTTGCAGGCACTTC-3′ | 186 | NM_007810.3 |
LHr | Forward: 5-TGAGTCCATCACGCTGAAAC-3′ Reverse: 5′-AGATTAGCGTCGTCCCATTG-3′ | 80 | NM_013582.2 |
β-Actin | Forward: 5′-CACACCTTCTACAATGAGCTGC-3′ Reverse: 5′-CATGATCTGGGTCATCTTTTCA-3′ | 108 | NM_007393.5 |