Fig. 3

A bioimaging probe targeting the interphase. a The design of fluorescent probes that specifically detect a sialic acid residue. The manufacturing process is schematically described in the upper panel. Nanoparticles were composed of 2-methacryloyloxyethyl phosphorylcholine (MPC)-based polymers namely poly(MPC)-co–n-butyl methacrylate (BMA)-co–o-nitrophenyloxycarbonyl polyethylene glycol methacrylate (MEONP)-co-p-methacryloxyethyl thiocarbonyl rhodamine B (MTR). This PMBNR probe is biocompatible because of the coating with MPC polymers. Reproduced from [19]. Reprinted with permission from Cho et al. [19]. Copyright 2014 American Chemical Society. b Visualization of sugar chains in the interphase. LLC-PK1 cells coexpressing α and β subunits of H⁺,K⁺-ATPases were treated with the fluorescent probes described in a. Note that the β subunit is glycosylated and contains sialic acid groups. Left panel shows a representative example of a vertical scan image of the cell. Right panel is a schematic illustration demonstrating the binding of the probes to the sugar chains of the β subunit