Fig. 3

p62 expression changes in rat myocardium. a, b p62 immunofluorescence expression in cardiomyocytes. Immunoreactive regions of each group were stained bright red. Few immunoreactivity differences among groups can be observed except that the EEP group showed significantly more than the C group. Original magnification ×400; bar 20 μm. b The average integral optical density (AIOD) value of p62 immunoreactive image analysis in cardiomyocytes indicates that the difference in the EEP group was significantly different compared with the C group, *P < 0.05. The EEP + E group compared with the EE group was significantly different, ^§ P < 0.05. The LEP, EEP + E and LEP + E groups were significantly different compared with the EEP group, ^† P < 0.05. c p62 protein expression changes in EP and EP-induced cardioprotection, detected by immune blotting. In the EEP group compared with the C group, the difference was significant, *P < 0.05. In the EEP + E group and LEP group compared with the EEP group, the difference was significant, ^† P < 0.05. C control, EE exhaustive exercise, EEP early exercise preconditioning, LEP late exercise preconditioning, EEP + E early exercise preconditioning plus exhaustive exercise, LEP + E late exercise preconditioning plus exhaustive exercise (color figure online)