Fig. 6
Identification of mRNA expression of α-adrenoceptor isoforms α1A, α2A, α2B, and α2C in the masseter muscle and lower lip using reverse transcription-PCR. a Typical examples of the amplified PCR fragments were consistent with the length predicted by the subunit primers. All PCR products were compared with a 100-base DNA ladder (Φ). G Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). b Gene expression of the α-adrenoceptor isoforms in the masseter muscle (black bars) and lower lip (white bars) normalized with GAPDH from the same template and shown as a ratio (mean ± SEM). Statistical significance of the differences between the masseter muscle and lower lip was determined by ANOVA, followed by a post hoc test (Fisher’s PLSD). *P < 0.05, **P < 0.01 vs. masseter muscle