Fig. 4

MALAT-1 elevated Livin levels by enhancing the stability of the Livin protein. 786-O and Caki-1 cells were transfected with si-MALAT-1 or an si-control, respectively. Livin mRNA (a) and Livin protein (b) levels in the 786-O and Caki-1 cell lines were detected by qRT-PCR and a Western-blot analysis, respectively. c An RNA pull-down assay was performed, as described in the experimental procedures. MALAT-1 or antisense RNA (NC) was incubated with cell extracts, and the Livin protein was assayed by Western blot. d RIP experiments were performed using a Livin antibody or nonspecific IgG, and specific primers were used to detect MALAT-1. MALAT-1-overexpressed 786-O cells were treated with CHX and MG132. After incubation for 4 h, Livin protein expression was detected by a Western-blot analysis (e)