Fig. 3
Expression of CNP in myocardial tissue. a Immunohistochemistry staining of CNP in cardiomyocytes. All of the groups with CNP immunostaining demonstrated a brown granular pattern of distribution (original magnification ×400). b Quantitative analysis of CNP immunostaining. The CNP immunoreactive density values in Group HE, Group EEP and Group LEP were significantly higher than those in Group C (p < 0.05); compared with Group HE, the CNP immunoreactive density values in Group EEP+HE and Group LEP+HE increased significantly (p < 0.05). c Myocardial CNP was determined by western blot analysis. The CNP levels in Group HE, Group EEP and Group LEP were significantly higher than those in Group C (p < 0.05); compared with Group HE, the CNP levels in Group EEP+HE and Group LEP+HE increased significantly (p < 0.05). d Plasma CNP levels were measured by an immunosorbent assay. The plasma CNP levels in Group EEP+HE and Group LEP+HE were significantly higher than those in Group C (p < 0.05). The data are presented as the mean ± SD. *p < 0.05 vs Group C, # p < 0.05 vs Group HE