Fig. 5

Whole-cell patch-clamp recordings in HEK293T cells expressing TRPM7 alone or TRPM6 with TRPM7: a Representative time trace of membrane current from mock-transfected HEK293T cell. A small endogenous TRPM7-like currents were observed (n = 5). b Representative time trace from HEK293T cells expressing mouse TRPM7 alone. The current size was approximately ten-times larger compared to the endogenous currents, and these currents were inhibited by 0.1, 0.5, or 2.0 mM 2-APB (n = 7). The outwardly rectifying current–voltage relationship is shown on the right. c Representative time trace from HEK293T cells co-expressing mouse TRPM6 with TRPM7. The current was activated by 0.1, 0.5, or 2.0 mM 2-APB (n = 7). d Dose-dependent modification by the application of 2-APB at +110 mV. Currents from TRPM7 alone were inhibited, while currents of TRPM6 with TRPM7 were activated by 2-APB. These inhibitions or activations were statistically significant (**p = 0.013, n = 4; *p = 0.002, n = 7, one-way ANOVA with Bonferroni post hoc test). Red and black dots in the traces indicate the point at which I–V curves were generated