Fig. 1

The effects of estrogen on RIP140 expression in OB, BMSCs, and osteoclasts. a, b RIP140 protein and mRNA expression were respectively measured by Western blot and qRT-PCR in OB exposed to 10⁻⁸ M estrogen for 2 days. n = 3. c, d RIP140 protein and mRNA expression were respectively measured by Western blot and qRT-PCR in BMSCs exposed to 10⁻⁸ M estrogen for 2 days. n = 3. e, f RIP140 protein and mRNA expression were respectively measured by Western blot and qRT-PCR in mature osteoclasts exposed to 10⁻⁸ M estrogen for 2 days. n = 3, *p < 0.05, **p < 0.01. g, h The effects of 10⁻⁸ M estrogen on RIP140 protein and mRNA expression were respectively measured by Western blot and qRT-PCR during osteoclasts differentiation process at different time course. n = 3, *p < 0.05, **p < 0.01. OB osteoblasts, BMSCs bone marrow mesenchymal stem cells, Con control, E2 17β-estradiol