Fig. 2

The phosphatase inhibitor calyculin A near maximally stimulates basal I _Ca,L in FVMs. a The cell was exposed to high concentration of Cal A (Cal A’, 100 nM) and then to ISO (1 µM). b The myocyte was superfused with ACh (10 µM) to completely inhibit AC activity and then to high concentration of Cal A as in a. c The myocyte was superfused with low concentration of Cal A (Cal A″, 1 nM) and then to IBMX (100 µM). d Bar graphs representing the average effects of Cal A, alone and together with ISO or IBMX, on I _Ca,L. **p < 0.01; ***p < 0.005, compared with basal I _Ca,L