Fig. 6

Comparison of the inhibitory effects of capsaicin on the currents of WT hTRPM8 and hTRPM8 mutant Y745H expressed in HEK293T cells. a, b Representative traces of the cold-activated currents (upper) in the absence and presence of capsaicin (100 μM) with temperature changes (lower) in HEK293T cells expressing WT (a) and Y745H (b). c, d Whole-cell current–voltage relationships of WT (c) and Y745H (d) expressed in HEK293T cells in the absence and presence of capsaicin at the points indicated by asterisk in the traces (a, b). e, f Comparison of the current densities activated by cold in the absence and presence of capsaicin (100 μM) at −60 mV (e) and +100 mV (f). **p < 0.01. n = 8–12. g Comparison of the ratios of the cold-activated currents at +100 mV without and with capsaicin from the data shown in f. **p < 0.01. Data are shown as the mean ± SEM